The cluster of GABA receptor genes (GABRB3

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Of note, MCA205 fibrosarcomas almost completely lost nuclear HMGB1 expression (Figures 1a and b), whereas CT26 carcinomas conserved nuclear HMGB1 in 40 of malignant cells (Figures 1a and b). This staining was strongly reduced upon transfection with an siRNA targeting HMGB1, confirming the specificity of the immunohistochemical method (Figures 1c and d). We confirmed the relative and progressive loss of nuclear HMGB1 in human BCs by investigating the nuclear staining using the specific anti HMGB1 Ab (Figure 1e).